Amino Acid Analyser ARACUS, MembraPure, Germany
The ARACUS is a dedicated amino acid analyser for high end analysis of amino acids in research, food quality control (own patent 10 2016 010 887), beverages and feedstuff and in the clinical laboratory. It combines innovative technologies with the classic routine analysis of amino acids by post-column derivatization with ninhydrin and the detection at 440 nm and 570 nm using maintenance-free LED photometers. The setup of the instrument allows the continuous analysis of 96 samples (2 x 48 vials) in a cooled autosampler. The pump allows the simultaneous operation of two fluid lines (eluent line and reactive line), which guarantees a constant mixing. Eluents and reagent solution are selectively chosen through valves and are ideally mixed to each other. The addition of ninhydrin provides a quantitative analysis statement. A washing routine of the injection valve and syringe pump avoids cross contaminations.The level of the individual reagents can be monitored during the runs and warns the user if the quantity is no longer sufficient.
The data acquisition and analysis software aminoPeak or Clarity records two analogue channels simultaneously (570 nm, 440 nm). Chromatograms are shown online. Internal databases allow fast peak identification. Quantitative calculations are performed with internal or external standards, dilution and/or multiplication factors. Several analysis runs can be compared using compiler programmes. The results of each analysis are documented in an individual report.
The concept behind the ready-to-use eluent and reagent kits allows the reproducible analysis of amino acid samples. Each kit is produced under standardised procedures, tested and certified before shipping. Depending on the application, the eluent and reagent kit contains the necessary amount for about 500 runs. Kits and columns are optiomally matched to each other.
Method: post colum derivatization with ninhydrine
Spectroscopic detection: LED photometers: 440 nm + 570 nm
Detection limit: < 2 nmol/mL
Reproducibility of retention time: < 0.3 % RSD
Reproducibility of area: ca. 1 % RSD
2 pump system allows to operate with a lower noise level and are required for OPA post column derivatization method.
Sensitive fluorescent detection reagent for amines with o-phtalaldehyde (OPA). Configuration ARACUS advanced is needed.
Active control of fluid volume by weight cell of each bottle
IQ and OQ documents
prepared documents for the IQ and OQ procedure. Validation support can also be requested additionally.
It supplies the main unit and eluent box with a protective gas atmosphere by reducing the presence of atmospheric oxygen to enhance the signal to noise ratio.
software 21 CFR part 11 compliance
As an option, Clarity can be used if 21 CFR Part 11 conformity is required for manual and automatoc peak integration with integreated IQ/OQ procedure for software.
InVitro diagnostic Kit according to DIN ISO 13485
IVD kits for the qualitative and quantitative determination of amino acids in physiological fluids, for in vitro diagnostic use with ARACUS amino acid analyser.
Determination of free/ physiological amino acids in blood plasma, serum, salvia and urine newborn screening
– Argininemia (ARG)
– Arginosuccinic aciduria (ASA)
– Citrullinemia (CIT)
– Homocystinuria (HCY)
– Maple syrup urine disease (MSUD)
– Phenylketonuria (PKU)
– Ornithine transcarbamylase deficiency (OTC)
– Tyrosinemia (TYR)
Determination of hydrolysed amino acids in food, beverages and feedstuff
– fish feed
Determination of biogenic amine for the investigation of metabolic processes plant
– growth factor: Tryptamine
Animal & human
– neurotransmitter: Histamine, Serotonin, Dopamine etc.