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Amino Acid Analyzer, Artemis 6000

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Artemis 6000 Amino Acid Analyzer (AAA), SCION Instruments, UK

The SCION Artemis 6000 Amino Acid Analyzer is as a turn-key solution complete with a sophisticated HPLC system, ready-to-use buffers, reagents and columns. The Artemis 6000 combines the advantages of the classical ion exchange separation methods with the modern techniques of HPLC. Specifically configured and designed for routine or research amino acid determination, the SCION Artemis 6000 is the complete Amino Acid Analyzer to today’s modern laboratory.

Description

Turn-key solution with reliable performance

∙ Based on SCION’s 50+ years experience in chromatography, results in unmatched performance
∙ Configured package for various applications, e.g. protein hydrolysate, physiological fluid, etc.
∙ Pre-tuned and recommended conditions for “Inject and Run”
∙ Excellent sensitivity with a detection limit as low as 8 pMol (Asp 3p Mol), with great separation power: average peak resolution>1.5, all peak resolution >1.2 (hydrolysate AA) or >1.0 (free AA)

Ease of use

∙ Fully controlled by 21 CFR part 11 compliance data system with multi-language support
∙ Visualised LCD with direct control and support method creation, editing and saving
∙ Modular design, ease of maintenance

Productivity & Robust

∙ Fully automated analysis with protection, e.g. auto washing, leakage protection, etc.
∙ Rapid analysis: 30 – 50 min for hydrolysate sample, 70 – 180 min for physiological sample
∙ Outstanding repeatability in both retention time and peak area
∙ Long-term stability with replaced buffer and consumables
∙ High efficient separation column and ammonia filter column with long lifetime
∙ Inert and non-metal materials for pipes contact with buffer solutions & samples to avoid interference
∙ All buffers protected by inert gas, store in reagent organizer with refrigeration

Oil and Gas

How the SCION AAA can work for the Oil & Gas market

SCION Artemis 6000 Amino Acid Analyzer is designed to operate 24/7, a definite requirement for around the clock amino acid processing. Amino Acid concentration in oil rock is one of the most important identifiers to determine the quality of the rocks (rich or lean). Thus, the Amino Acid Analyzer is frequently employed for oil and gas exploration.

Chemical

How the SCION AAA can work for the chemical market

The SCION Amino Acid Analyzer is an ideal solution for any chemical laboratory.  Routinely used in the leather industry, the Amino Acid Analyzer is used to determine the concentration of hydroxyproline which affects the quality of the leather.

Pharmaceutical

How the SCION AAA can work for the Pharmaceutical market

SCION Artemis 6000 Amino Acid Analyzer is a versatile technique commonly used in the pharmaceutical industry. For example, some Amino Acids are used for therapy, including Arginine, Aspartate, Cysteine, Glutamic Acid, Histidine and Methionine. Amino Acids are also the building blocks for synthesis of drugs, especially peptide based drugs.

Environmental

How the SCION AAA can work for the Environmental market

SCION Artemis 6000 Amino Acid Analyzer are designed to support detailed analysis of Amino Acids from recycled products. For example, production of Amino Acids, peptides or protein from industrial waste (like inedible fish, fish scale, oil cake, etc) can help us to reduce and recycle waste products to protect the environment.

Food

Food and agriculture are two of the most important application areas of Amino Acid Analysis.  In the food industry, especially the food production process and new product development, the nutrition value and taste are the most crucial factors. For example, in process improvement, a comparison of Amino Acids before and after the process changed in required to evaluate the new process. The analysis of Amino Acids for new product development is also crucial before launching in the market.

In agriculture, the main application of Amino Acid Analyzer is mainly for feedstuff analysis and thremmatology studies.

SCION Artemis 6000 Amino Acid Analyzer provides both Protein Hydrolysate (PH) and Physiological Fluids (PF) systems for the food industry. The PH system is mainly for feedstuff, including the oxidised protein hydrolysate specially optimised for sulfur containing Amino Acids such as Lysine, Threonine, Cystine and Methionine. The PF system is for determination of free Amino Acids in food/nutrition products. Both system are completed package with relevant buffer, reagent and columns together with verified method.

Technical Specifications

Refrigerated Reagent Organiser For storing all reagents, buffers and wash solutions. • Front side operated • Special valves for applying inert gas for oxygen-free storage • Integrated cooling • Transparent cover is equipped on top to avoid dust and for easy observation of buffer consumption Solvent Delivery System • Only inert materials come in contact with buffer solutions & samples (PEEK or PTFE) • Quaternary gradient pump with two-plunger for smooth eluent delivery • Dual plunger pump with special design for low pulsation (less than 1 %) • Flow rate of 0.000 to 9.999 ml/min • Flow rate stability with RSD<0.1% • Maximum pressure up to 400 bar (6000 PSI) • Integrated 4-channel vacuum degasser • Battery buffered programs stored for the amino acid determination (hydrolysates and physiological fluids) • More than 100 programmable gradient steps with a resolution of 0.1% • Graphic display of gradient profile A, B, C, and D • Programmable mixing cycle for the buffer solution • Programmable flushing procedure • Extended diagnostic features (e.g. high pressure control, low pressure control) Autosamplers • For automatic injection of samples. All parts which come in contact with liquids are chemically inert and biocompatible (PEEK or PTFE) • Large graphical display and keyboard for easy control • Programmable washing procedure with selectable volumes • Programmable port for purging Injection mode Loop overfill mode or Variable sample dosage Injection volume 0-500 µL, 1 μl increments Reproducibility RSD<1% upon injection of 10 µL variable volume Carry over <0.01% Temperature control Temperature controlled sample compartment (5 – 70° C) Sampling Operating in x, y and z-axis Sample rack Two sample racks, 120 vials capacity Interface Large graphical display and keyboard Analytical Specifications Analysis time Hydrolysate sample: 30 – 50 min Physiological sample: 70 – 180 min Sensitivity <8 pMol (All) <2.5 pMolASP, 3 pMol) Reproducibility Qualitative: – RSD<0.5% (All Amino Acids) – RSD<0.1% (Arg) Resolution Hydrolyzate sample – >1.2 (All) or >3.3 (Average), 150 mm column – >1.4 (All) or >3.5 (Average), 175 mm column Free amino acids – >1.0 (All) or >2.5 (Average) Bioamine – >1.2 (All) or >3.0 (Average) Amino Acid Reaction Module • Integrated 2-plunger reagent dosing pump with adjustable flow rate (0.01 to 2.0 ml/min) • Built-in dual filter photometer (440 and 570 nm) with constant signal output and signal summary option • Programmable signal offset • Three different risetimes selectable • Temperature controlled column oven (20 to 99° C ±1° C) with active cooling capability • Temperature controlled post-column derivatisation reactor (up to 180° C ±1° C) • Automatic valve for coil flushing • Display of the actual system pressure • Safety features (e.g. leakage of reactor and column, high pressure) Software • Complete control of Gradient Program, Column & Reactor Temperature • Programmable Sample Sequence with individual run times, gradient and temperature profiles • Automatic usage control of Buffer & Reagent solutions • Emergency-Program (SOS) feature in case of pressure errors • Manual control of each single unit Consumables • Ready-made buffer solutions (each lot tested individually) • Separation columns for a variety of applications with long term stability • Ready-made Ninhydrin solution • A variety of standard solutions Installation Parameters Dimension 720mmx580mmx880mm (W X D X H) Weight 110kg Power Supply 215V – 240V Power Consumption Max 750W Environment Humidity: <90% (Relative) Temperature: 5°C to 35°C N2 gas supply Purity >99.99%, Output pressure 1-3 bar (0.1Mpa – 0.3 Mpa)

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