Elite Scientific & Meditech CO.

HPLC System

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The Agilent 1200 Series HPLC System is a must have for an analytical lab. The modular and compact design of the HPLC System allows users to configure to their needs while taking up a minimal amount of bench space. This HPLC system features a powerful combination of speed, resolution, and sensitivity with a high throughput and improved quality of results. The 1200 series HPLC system uses a plug and play modular system, so modules automatically hand shake with each other, or can be used separately depending on your needs. The Agilent 1200 Series HPLC instrument uses a dual lamp design and temperature control through diode array detectors to bring you outstanding sensitivity. This is also partnered with a high pressure binary pump for flexible solvent delivery, and a degasser for stable flow rate. The thermostat auto sampler of this high-performance liquid chromatography machine has a temperature range of between 4-40°C, as well as a high-performance auto sampler with an injection range of between 0.1-100 µl.

The Agilent 1200 Series HPLC System consists of a G1379B Vacuum Degasser, a G1312A Binary Pump, a G1329A Autosampler, a G1330B ALS Thermostat, and a G1315D DAD Detector. The Diode Array Detector allows for flexible slit settings for a range of 1 to 16 nm, letting you customize the desired sensitivity, linearity and spectral resolution. This HPLC System, built for purification and analysis, also comes with a controlling PC installed with Chemstation software to help manage and interpret large amounts of data collected from the system. TheChemstaion software is windows based, and user friendly and gives you level 5 control of your instrument with built in diagnostics and early warnings for maintenance. Thanks to the scalability and easy maintenance of this LC System, this work horse will rise to meet your needs for years to come.

Agilent 1200 Series HPLC System Specifications:

Thermostated Autosampler
Temperature Range 4-40°C in 1° increments
DAD Detector
Detection type 1024-element photodiode array
Light source Deuterium and tungsten lamps
Wavelength range 190 – 950 nm
Short term noise < ± 0.8 x 10 -5 AU at 254 nm and at 750 nm
Drift < 0.9 x 10 -3 AU/hr, at 254 nm
Linear absorbance range > 2 AU (upper limit)
Wavelength accuracy ± 1 nm Self-calibration with deuterium lines, verification with holmium oxide filter
Wavelength bunching 1 – 400 nm Programmable in steps of 1 nm
Slit width 1, 2, 4 , 8, 16 nm Programmable slit
Diode width  < 1 nm
Flow cell Standard 13-µL volume, 10 mm cell path length, 120 bar (1760 psi) pressure maximum
Vacuum Degasser
Flow rate 0-5 ml/min per channel 

5-10 ml/min at reduced degassing performance

Number of channels 4
Internal volume per channel Typically 1 ml per channel
Materials in contact with solvent PTFE – PEEK
pH range 1 – 14
Analog output (AUX) For pressure monitoring, range 0 – 3 V
Binary  Pump
Hydraulic system Dual plunger in series pump with proprietary servo-controlled variable stroke drive, floating plungers and active inlet valve
Setable flow range 0.001 – 5 ml/min, in 0.001 ml/min increments
Flow range 0.1 – 5.0 ml/min
Flow accuracy ± 1% or 10 µl/min whatever is greater
Pressure Operating Range Setpoints 0.001 – 5 ml/min, in 0.001 ml/min increments
Pressure pulsation < 2 % amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa
Recommended pH range 1.0 – 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
ALS Auto Sampler
Injection range  0.1 – 100 µl in 0.1 µl increments
Replicate injections 1 – 99 from one vial
Precision < 0.25% RSD from 5 – 100 µl, < 1% RSD 1 – 5 µl variable volume
Minimum sample volume 1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvial
Carryover Typically < 0.1%, < 0.05% with external needle cleaning
Sample viscosity range 0.2 – 50 cp
Replicate injections per vial 1 – 99
Sample capacity 100 × 2-ml vials in 1 tray 40 × 2-ml vials in ½ tray 15 × 6-ml vials in ½ tray Agilent vials only)
Injection cycle time Typically 50 s depending on draw speed and injection volume
General
Dimensions 14x18x33″
Power 100-120/220-240 V, 50/60 Hz

Included

  • G1379B Vacuum Degasser
  • G1312A Binary Pump
  • G1315D DAD Detector
  • G1329A Autosampler
  • G1330B ALS Thermostat
  • Solvent Tray
  • Windows 7 Notebook with Chemstation
  • System Validation Report with Clean Bill of Health
  • Operator’s Guide
  • CAN Communication Cables
  • 120V Power Cords
  • 30-Day Warranty
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